The Analysis of Genetic Polymorphism. The Relationship between Interleukin – 4 Polymorphisms and Intraepithelial Cervical Neoplasia

Authors

  • Ioana ROTAR “Iuliu Haţieganu” University of Medicine and Pharmacy Cluj-Napoca, Ist Obstetrics and Gynecology Department, 13 Emil Isac, 400023 Cluj-Napoca, Romania.
  • Sorana D. BOLBOACĂ “Iuliu Haţieganu” University of Medicine and Pharmacy Cluj-Napoca, Department of Medical Informatics and Biostatistics, 13 Emil Isac, 400023 Cluj-Napoca, Romania.
  • Daniel MUREŞAN “Iuliu Haţieganu” University of Medicine and Pharmacy Cluj-Napoca, Ist Obstetrics and Gynecology Department, 13 Emil Isac, 400023 Cluj-Napoca, Romania.
  • Radu POPP “Iuliu Haţieganu” University of Medicine and Pharmacy Cluj-Napoca, Department of Medical Genetics, 13 Emil Isac, 400023 Cluj-Napoca, Romania.
  • Marius FĂRCAŞ “Iuliu Haţieganu” University of Medicine and Pharmacy Cluj-Napoca, Department of Medical Genetics, 13 Emil Isac, 400023 Cluj-Napoca, Romania.
  • Felicia PETRIŞOR “Iuliu Haţieganu” University of Medicine and Pharmacy Cluj-Napoca, Department of Medical Genetics, 13 Emil Isac, 400023 Cluj-Napoca, Romania.
  • Cristina BUTUZA “Iuliu Haţieganu” University of Medicine and Pharmacy Cluj-Napoca, Ist Obstetrics and Gynecology Department, 13 Emil Isac, 400023 Cluj-Napoca, Romania.
  • Gabriela CARACOSTEA Ist Obstetrics and Gynecology Clinic, Emergency County Hospital Cluj-Napoca, 3-5 Clinicilor, 400006 Cluj-Napoca, Romania.
  • Florin STAMATIAN “Iuliu Haţieganu” University of Medicine and Pharmacy Cluj-Napoca, Ist Obstetrics and Gynecology Department, 13 Emil Isac, 400023 Cluj-Napoca, Romania.

Keywords:

Genetic polymorphism, Restriction fragment length polymorphism (RFLP), Polymerase chain reaction (PCR), Interleukin-4 (IL4), Cervical intraepithelial neoplasia (CIN), Human papiloma virus (HPV) infection, Human papiloma virus high risk (HPV-HR), Negative

Abstract

Objectives: Interleukin 4 plays a critical role in T helper 2 responses to HPV infection and angiogenesis. The present study aim to study the association between the IL4 promoter polymorphism – 590 C>T, respectively VNTR intron 2 polymorphism and cervical intraepithelial neoplasia. Material and Method: We have realized a prospective case controls study that included 128 cases of intraepithelial neoplasia positive for HPV HR testing and 111 controls negative for intraepithelial lesion and also negative for HPV HR. Clinical examination was performed on each patient; blood and cervical sample were obtained. Cervical probes were analyzed regarding cytology and HPV HR testing. From peripheral blood DNA sample was obtain followed by genotype analysis for IL4 -590 C>T using PCR RFLP, respectively IL4 70 bp VNTR determined by PCR. Results: The absolute frequency of genotypes for IL4 -590 C>T was T/T-5, C/T-42, C/C-81 in the cases group respectively T/T-2, C/T-32, C/C-77 in the control group. The chi-square test had a value of 0.983 (p=0.321) while considering the presence of a minimum one single variant allele as a risk factor for cervical cancer, respectively 0.926 (p=0.336) for homozygous variant genotype. Odds ratio was 0.761 (95%CI [0.443-1.306]) while considering C/T+T/T respectively 2R/3R, 2R/2R as a risk factor, and 0.451 (95%CI 95% [0.086-2.374]) - TT respectively 2R/2R as a risk factor. Conclusion: No linear statistical significant association has been found between IL4 polymorphism and cervical neoplasia (p = 0.322).

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How to Cite

1.
ROTAR I, BOLBOACĂ SD, MUREŞAN D, POPP R, FĂRCAŞ M, PETRIŞOR F, BUTUZA C, CARACOSTEA G, STAMATIAN F. The Analysis of Genetic Polymorphism. The Relationship between Interleukin – 4 Polymorphisms and Intraepithelial Cervical Neoplasia. Appl Med Inform [Internet]. 2011 Jan. 18 [cited 2024 Jun. 22];27(3):43-52. Available from: https://ami.info.umfcluj.ro/index.php/AMI/article/view/34

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