Inflammation and Oxidative Stress in Young and Aged Rats after Acute Homocysteine Administration

Authors

  • Cristina Sorina CĂTANĂ Department of Medical Biochemistry, “Iuliu Hatieganu” University of Medicine and Pharmacy, 13 Emil Isac, 400023 Cluj-Napoca, Romania
  • Elena Cristina CRĂCIUN Department of Pharmaceutical Biochemistry and Clinical Laboratory, “Iuliu Hatieganu” University of Medicine and Pharmacy, 13 Emil Isac, 400023 Cluj-Napoca, Romania
  • Maria DRONCA Department of Medical Biochemistry, “Iuliu Hatieganu” University of Medicine and Pharmacy, 13 Emil Isac, 400023 Cluj-Napoca, Romania
  • Victor CRISTEA Immunology Department, “Iuliu Hatieganu” University of Medicine and Pharmacy, 13 Emil Isac, 400023 Cluj-Napoca, Romania
  • Claudia GHERMAN The Oncology Institute „Prof. Dr. Ion Chiricuţă”, 34-36 Republicii, 400015 Cluj-Napoca, Romania.
  • Tibor Ludovic KRAUSZ Immunology Department, “Iuliu Hatieganu” University of Medicine and Pharmacy, 13 Emil Isac, 400023 Cluj-Napoca, Romania
  • Cristina DRUGAN Department of Medical Biochemistry, “Iuliu Hatieganu” University of Medicine and Pharmacy, 13 Emil Isac, 400023 Cluj-Napoca, Romania
  • Tudor DRUGAN Medical Informatics and Biostatistics, “Iuliu Hatieganu” University of Medicine and Pharmacy, 13 Emil Isac, 400023 Cluj-Napoca, Romania

Keywords:

hyper-homocysteinemia, aging, cytokines, GPx, oxidative stress

Abstract

Introduction: Hyperhomocysteinemia plays an etiologic role in homocystinuria, neurodegenerative and cardiovascular diseases. Potential mechanisms involved in the degenerative diseases of aging include: oxidative stress, endothelial dysfunction and inflammation. Aim: In the present study we evaluated the effect of acute administration of homocysteine (Hcy), at a level similar to that found in homocystinuria, on biochemical markers of inflammation (such as IL-6) and of oxidative stress (such as gluthathione peroxidase - GPx). Material and Method: The study was performed on 40 young and older Wistar rats. IL-6 serum level was quantified by a high-sensitivity enzyme-linked immunoabsorbent assay (ELISA) method and the activity of whole blood GPx was measured using a commercially available Randox kit. Results: Our results showed that Hcy administration increased the pro-inflammatory cytokine IL-6 in young rats (p<0.3) and decreased IL-6 level in older rats (p<0.008), when compared to the control group. GPx activity was found to increase with age (587.07 U/gHb versus 847.5 U/gHb, p<0.001). Two hours after Hcy administration, GPx activity was found to decrease, but not in a statistically significant manner. The difference between GPx activities in Hcy treated groups remains statistically significant (p<0.01) in the younger group, compared to older group (556.62 U/gHb versus 748.38 U/gHb). Conclusion: Our results indicate the existence of a correlation between hyperhomocysteinemia, proinflammatory state and oxidative stress, illustrated by the direct dependence of whole blood GPx activities on the increasing age.

Author Biographies

Cristina Sorina CĂTANĂ, Department of Medical Biochemistry, “Iuliu Hatieganu” University of Medicine and Pharmacy, 13 Emil Isac, 400023 Cluj-Napoca, Romania

Biochemistry

Elena Cristina CRĂCIUN, Department of Pharmaceutical Biochemistry and Clinical Laboratory, “Iuliu Hatieganu” University of Medicine and Pharmacy, 13 Emil Isac, 400023 Cluj-Napoca, Romania

Biochemistry

Maria DRONCA, Department of Medical Biochemistry, “Iuliu Hatieganu” University of Medicine and Pharmacy, 13 Emil Isac, 400023 Cluj-Napoca, Romania

Biochemistry

Victor CRISTEA, Immunology Department, “Iuliu Hatieganu” University of Medicine and Pharmacy, 13 Emil Isac, 400023 Cluj-Napoca, Romania

Immunology

Claudia GHERMAN, The Oncology Institute „Prof. Dr. Ion Chiricuţă”, 34-36 Republicii, 400015 Cluj-Napoca, Romania.

Oncology

Tibor Ludovic KRAUSZ, Immunology Department, “Iuliu Hatieganu” University of Medicine and Pharmacy, 13 Emil Isac, 400023 Cluj-Napoca, Romania

Pharmacology

Cristina DRUGAN, Department of Medical Biochemistry, “Iuliu Hatieganu” University of Medicine and Pharmacy, 13 Emil Isac, 400023 Cluj-Napoca, Romania

Biochemistry

Tudor DRUGAN, Medical Informatics and Biostatistics, “Iuliu Hatieganu” University of Medicine and Pharmacy, 13 Emil Isac, 400023 Cluj-Napoca, Romania

Informatics

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Published

15.06.2011

How to Cite

1.
CĂTANĂ CS, CRĂCIUN EC, DRONCA M, CRISTEA V, GHERMAN C, KRAUSZ TL, DRUGAN C, DRUGAN T. Inflammation and Oxidative Stress in Young and Aged Rats after Acute Homocysteine Administration. Appl Med Inform [Internet]. 2011 Jun. 15 [cited 2024 Jun. 21];28(2):15-21. Available from: https://ami.info.umfcluj.ro/index.php/AMI/article/view/176

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