Mobile Phase Optimization Method for Steroids Separation
The paper presented a mathematical model developed for optimization of mobile phase composition in thin layer chromatography applied on steroids separation. The proper solvents system was experimental identify as being a mixture of chloroform, cyclohexan, and methyl-ethyl-cetone. An original mathematical model was developed and used in order to identify the composition of mobile phase. Starting with the mathematical model and with the optimization procedure a computer program has been developed. The proposed model is able to optimize simultaneously many mobile phases with respect of the shortest analysis time and of the selectivity of compounds. The efficiency of the mathematical and optimization models is demonstrated on a sample of five androstane isomers.