TY - JOUR AU - MALUTAN, Andrei Mihai AU - COSTIN, Nicolae AU - CIORTEA, Razvan AU - MIHU, Dan PY - 2013/06/06 Y2 - 2024/03/29 TI - Variation of Anti-inflammatory Cytokines in Relationship with Menopause JF - Applied Medical Informatics JA - Appl Med Inform VL - 32 IS - 2 SE - Articles DO - UR - https://ami.info.umfcluj.ro/index.php/AMI/article/view/412 SP - 30-38 AB - <span style="font-family: Times New Roman; font-size: small;"> </span><p class="MsoNormal" style="margin: 0cm 0cm 0pt; text-align: justify;"><strong style="mso-bidi-font-weight: normal;"><span style="font-family: &quot;Garamond&quot;,&quot;serif&quot;; font-size: 11pt; mso-bidi-font-size: 12.0pt; mso-ansi-language: EN-GB;" lang="EN-GB">Aim. </span></strong><span style="font-family: &quot;Garamond&quot;,&quot;serif&quot;; font-size: 11pt; mso-bidi-font-size: 12.0pt; mso-ansi-language: EN-GB;" lang="EN-GB">The aim of this study was to assess serum levels of the key anti-inflammatory cytokines in women of reproductive age and in pre and postmenopausal women. <strong style="mso-bidi-font-weight: normal;">Material and Method.</strong> 175 women were enrolled and were divided into 5 groups (1 – Fertile women; 2 – Pre- and perimenopausal women; 3 – Postmenopausal women; 4 – Surgically-induced menopause; 5 – Chronic inflammation). Multiplex cytokine kits were used to evaluate serum levels of interleukin-4, -10 and -13. We determined the serum levels of follicle stimulating hormone, of luteinizing hormone, 17β-estradiol, progesterone, dehydroepiandrosterone and dehydroepiandrosterone sulfate using sandwich ELISA. <strong style="mso-bidi-font-weight: normal;">Results.</strong> IL-4, IL-10 and IL-17 present a statistically significant decrease (p=0.00, p=0.00, respectively p=0.0053) in women with natural or surgically induced menopause (groups 3 and 4), compared with fertile women and premenopausal women (Groups 1, 2 and 5). Serum levels of IL-4 and IL-10 are significantly higher in fertile patients with associated chronic inflammatory diseases (133.5±1.314 pg/ml, respectively 6.406±13.47 pg/ml) than in fertile patients without chronic inflammatory diseases or premenopausal women (84.67±1.22 pg/ml, respectively 0.627±0.714). <strong style="mso-bidi-font-weight: normal;">Conclusions. </strong>IL-4 and IL-10, together with IL-17, show significantly lower serum values in patients with natural or surgically induced menopause compared with patients of childbearing age or in premenopause. IL-4 and IL-10 show significantly higher serum values for patients of childbearing age presenting chronic inflammatory pathology compared with patients of childbearing age without chronic inflammatory pathology or premenopausal patients.</span><strong style="mso-bidi-font-weight: normal;"><span style="font-family: &quot;Garamond&quot;,&quot;serif&quot;; font-size: 11pt; mso-bidi-font-size: 12.0pt; mso-ansi-language: EN-GB;" lang="EN-GB"><span style="mso-tab-count: 1;"> </span></span></strong></p><span style="font-family: Times New Roman; font-size: small;"> </span><p class="MsoNormal" style="margin: 0cm 0cm 0pt;"><span style="font-family: &quot;Garamond&quot;,&quot;serif&quot;; font-size: 11pt; mso-bidi-font-size: 12.0pt; mso-ansi-language: EN-GB;" lang="EN-GB"> </span></p><span style="font-family: Times New Roman; font-size: small;"> </span> ER -