Microarray Data Normalization for the Analysis of an Experimental Design of Prostate Cancer Cells Treated with Genistein

Abstract

Oligonucleotide expression array technology has recently been adopted in many areas of biomedical research to measure the abundance of messenger ribonucleic acid transcripts for many genes simultaneously. We have analyzed multiple data sets from gene expression profiles of Genistein-treated PC3 prostate cancer cells experiments conducted in vitro. In the experimental design the PC3 cells were treated with Genistein in physiologically doses (T2=10uM) or therapeutically doses (T1=50uM) and compared with untreated controls. Resulting data sets from this experiment was analyzed with multiple mathematical and statistical tools in order to normalize and compare the results. The goal of our research was to prove that the gene expression of the enzymes apoptosis-related was statistical altered after the Genistein treatment.